Isogenic stem cell backgrounds
نویسندگان
چکیده
منابع مشابه
Generation of isogenic pluripotent stem cells.
The ability to reprogram somatic cell nuclei back into a pluripotent epigenetic state provides exciting new possibilities for in vitro research and cell transplantation therapy. There has been a significant quantity of recent research studies demonstrating that this epigenetic reprogramming process is possible with human and non-human primate somatic cells. In this review, various methodologies...
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Mutations in RAB39B are a known cause of X-linked early onset Parkinson's disease. Isogenic human embryonic stem cell lines carrying two independent deletions of RAB39B were generated using CRISPR/Cas9 genome editing tool. The deletions were confirmed by PCR and direct sequence analysis in two edited stem cell lines. Both cell lines showed pluripotency and displayed a normal karyotype. Further,...
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ژورنال
عنوان ژورنال: Nature Biotechnology
سال: 2011
ISSN: 1087-0156,1546-1696
DOI: 10.1038/nbt.1984